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marco  (R&D Systems)


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    Structured Review

    R&D Systems marco
    ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil <t>recruitment</t> <t>(CD45</t> + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + <t>MARCO</t> + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).
    Marco, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+marco/Mouse+MARCO+Fluorescein-conjugated+Antibody/pmc12288981-259-30-33
    Average 90 stars, based on 10 article reviews
    marco - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Csk-mediated Src family kinase regulation dampens neutrophil infiltration during pulmonary infection"

    Article Title: Csk-mediated Src family kinase regulation dampens neutrophil infiltration during pulmonary infection

    Journal: JCI Insight

    doi: 10.1172/jci.insight.188323

    ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).
    Figure Legend Snippet: ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).

    Techniques Used: Injection, Cell Counting, Infection, Comparison

    Related Articles

    Isolation:

    Article Title: Serum Amyloid A1 Induces Classically Activated Macrophages: A Role for Enhanced Fibril Formation.
    Article Snippet: .. Freshly isolated peritoneal cells or purified peritoneal macrophages and J774A.1 cells treated as described above, were harvested by scraping and stained with anti-F4/80 Frontiers in Immunology | www.frontiersin.org 3 (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD11b (Bio-Rad Laboratories, Feldkirchen, Germany), anti-MARCO (R&D Systems, Wiesbaden-Nordenstadt, Germany), anti-CD38 (BD Biosciences, San Jose, CA), anti-CD206 (BD Biosciences), or respective isotype controls for 20 min at 4°C. .. Flow cytometry was conducted on a FACSVerse instrument (BD Biosciences) and analysis was performed using FlowJo software (Ashland, OR).

    Article Title: Serum Amyloid A1 Induces Classically Activated Macrophages: A Role for Enhanced Fibril Formation
    Article Snippet: .. Freshly isolated peritoneal cells or purified peritoneal macrophages and J774A.1 cells treated as described above, were harvested by scraping and stained with anti-F4/80 (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD11b (Bio-Rad Laboratories, Feldkirchen, Germany), anti-MARCO (R&D Systems, Wiesbaden-Nordenstadt, Germany), anti-CD38 (BD Biosciences, San Jose, CA), anti-CD206 (BD Biosciences), or respective isotype controls for 20 min at 4°C. .. Flow cytometry was conducted on a FACSVerse instrument (BD Biosciences) and analysis was performed using FlowJo software (Ashland, OR).

    Purification:

    Article Title: Serum Amyloid A1 Induces Classically Activated Macrophages: A Role for Enhanced Fibril Formation.
    Article Snippet: .. Freshly isolated peritoneal cells or purified peritoneal macrophages and J774A.1 cells treated as described above, were harvested by scraping and stained with anti-F4/80 Frontiers in Immunology | www.frontiersin.org 3 (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD11b (Bio-Rad Laboratories, Feldkirchen, Germany), anti-MARCO (R&D Systems, Wiesbaden-Nordenstadt, Germany), anti-CD38 (BD Biosciences, San Jose, CA), anti-CD206 (BD Biosciences), or respective isotype controls for 20 min at 4°C. .. Flow cytometry was conducted on a FACSVerse instrument (BD Biosciences) and analysis was performed using FlowJo software (Ashland, OR).

    Article Title: Serum Amyloid A1 Induces Classically Activated Macrophages: A Role for Enhanced Fibril Formation
    Article Snippet: .. Freshly isolated peritoneal cells or purified peritoneal macrophages and J774A.1 cells treated as described above, were harvested by scraping and stained with anti-F4/80 (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD11b (Bio-Rad Laboratories, Feldkirchen, Germany), anti-MARCO (R&D Systems, Wiesbaden-Nordenstadt, Germany), anti-CD38 (BD Biosciences, San Jose, CA), anti-CD206 (BD Biosciences), or respective isotype controls for 20 min at 4°C. .. Flow cytometry was conducted on a FACSVerse instrument (BD Biosciences) and analysis was performed using FlowJo software (Ashland, OR).

    Staining:

    Article Title: Serum Amyloid A1 Induces Classically Activated Macrophages: A Role for Enhanced Fibril Formation.
    Article Snippet: .. Freshly isolated peritoneal cells or purified peritoneal macrophages and J774A.1 cells treated as described above, were harvested by scraping and stained with anti-F4/80 Frontiers in Immunology | www.frontiersin.org 3 (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD11b (Bio-Rad Laboratories, Feldkirchen, Germany), anti-MARCO (R&D Systems, Wiesbaden-Nordenstadt, Germany), anti-CD38 (BD Biosciences, San Jose, CA), anti-CD206 (BD Biosciences), or respective isotype controls for 20 min at 4°C. .. Flow cytometry was conducted on a FACSVerse instrument (BD Biosciences) and analysis was performed using FlowJo software (Ashland, OR).

    Article Title: Serum Amyloid A1 Induces Classically Activated Macrophages: A Role for Enhanced Fibril Formation
    Article Snippet: .. Freshly isolated peritoneal cells or purified peritoneal macrophages and J774A.1 cells treated as described above, were harvested by scraping and stained with anti-F4/80 (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD11b (Bio-Rad Laboratories, Feldkirchen, Germany), anti-MARCO (R&D Systems, Wiesbaden-Nordenstadt, Germany), anti-CD38 (BD Biosciences, San Jose, CA), anti-CD206 (BD Biosciences), or respective isotype controls for 20 min at 4°C. .. Flow cytometry was conducted on a FACSVerse instrument (BD Biosciences) and analysis was performed using FlowJo software (Ashland, OR).

    Article Title: Tissue macrophages and interferon-gamma signalling control blood-stage Plasmodium chabaudi infections derived from mosquito-transmitted parasites
    Article Snippet: Cells were then resuspended in PBS containing 2 % FBS and 3 mM EDTA (staining buffer) and seeded in 96-well V-bottom plates and incubated with anti-mouse CD16/CD32 (Fc block, BD Biosciences). .. After washing once in staining buffer, cells were stained with Live/DeadTM Fixable Blue Dead Cell Stain kit (ThermoFisher) or Zombie UVTM Fixable Viability Kit (Biolegend) and different combinations of the following fluorochrome-labelled antibodies (Biolegend unless otherwise specified) in staining buffer or Brilliant Stain Buffer (BD Biosciences): anti-CD3e (PE), anti-CD8α (BB515, BD Biosciences), anti-CD4 (BUV805, BD Biosciences), anti-NK1.1 (BV711 or PE), anti-γδTCR (APC), anti-CD19 (PE), anti-Ter119 (PE), anti-F4/80 (APC/Cy7), anti-CD11b (PE/dazzle 594), anti-CD169 (BV605 or PE/Cy7), anti-CCR2 (BV421), anti-MHC class II (I-A/I-E, BV510 or AF488), anti-CD11c (BV785 or biotin/SA-BV570), anti-Ly6C (BV711 or BV785), anti-Ly6G (BUV395, BD Biosciences), and anti-MARCO (APC, R&D Systems). .. Cells were washed twice with staining buffer and either acquired immediately or fixed with 2 % formaldehyde (Alfa Aesar) and stored in staining buffer at 4 ° C until acquisition .

    other:

    Article Title: Peptide conjugated particles
    Article Snippet: Antibodies against MARCO, SIGN-R1 and SIGLEC-1 (R&D Systems, MN, USA), CD68 (Abeam, Mass., USA) and Ki67 (Abeam), were used as indicated.

    Article Title: Peptide conjugated particles
    Article Snippet: Antibodies against MARCO, SIGN-R1 and SIGLEC-1 (R&D Systems, MN, USA), CD68 (Abeam, MA, USA) and Ki67 (Abeam), were used as indicated.



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    R&D Systems marco
    ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil <t>recruitment</t> <t>(CD45</t> + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + <t>MARCO</t> + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).
    Marco, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+marco/Mouse+MARCO+Fluorescein-conjugated+Antibody/pmc12288981-259-30-33
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).

    Journal: JCI Insight

    Article Title: Csk-mediated Src family kinase regulation dampens neutrophil infiltration during pulmonary infection

    doi: 10.1172/jci.insight.188323

    Figure Lengend Snippet: ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).

    Article Snippet: Alveolar macrophages were gated as CD45 + CD64 + F4/80 + MARCO + SiglecF hi using antibodies against CD45 (clone 30-F11, BioLegend), CD64 (clone X54-5/7.1, BioLegend), F4/80 (clone BM8, BioLegend), MARCO (clone 579511, R&D Systems), and SiglecF (clone S17007L, BioLegend).

    Techniques: Injection, Cell Counting, Infection, Comparison